Eurasian Journal of Veterinary Sciences - 2013; 29(1)

Investigation of OLR1 gene polymorphism of various cattle breeds in Turkey

Gözde Yazıcıtunç, Mehmet Nizamlıoğlu — 2013-03-01

Aim: The objective of this study was investigation of OLR1 gen polymorphism among different cattle breeds in Turkey. Materials and Methods: A total of 147 blood samples were collected from Anatolian Grey (AG), Anatolian Black (AB) and South Anatolian Red (SAR) cattle breeds and DNAs were isolated. PCR-RFLP method was used to determine allele types. Genotype and allele frequencies were calculated after genotyping. Results: Results are evaluated by square test (χ2). The significance level was taken as 0.05. While AG cattle breed deviated from HW equilibrium, SAR and AB cattle breeds were on the HW equilibrium and they are similar to each other. Conclusion: It is thought that the effect of polymorphic region in OLR1 gene on milk fat and the ratio of milk fat will be more efficient by studying with various polymorphic areas, and by using other cattle races that they have effects on similar characteristics of milk.

Factors effecting live weight, body measurements, disease incidence, livability and fertility of sport horses: I. Broodmares fertility and disease incidence, livabilityand first born knowledges in foals

Deniz Taşkın, Serdar Koçak — 2013-03-01

Aim: The aim of this research was to determine that the factors effecting first born knowledges, disease incidence, livability and fertility in sport horses. Materials and Methods: The material of this study includes 42 head sport and Friesian mares and 118 head foals in the years 2002-2009. Results: Gestation length sport and the Friesian horses were 331.36±1.71 and 326.04±1.41 days. Effects of breed, age and month of birth on gestation length were not significant. Pregnancy rates in KWPN, others and Friesian were 90.9, 80.0 and 85.7%, foaling rates 77.3, 60.0 and 71.4 %, respectively. The disease incidence were 32.1; 77.8 and 100.0 % for males and 81.5, 73.3 and 53.8 % for females in KWPN, others and Friesian foals until twelve months of age. Foals livability of KWPN, others and Friesian foals were 96.4, 100.0 and 82.4 % for male, 88.9, 86.7 and 100.0 for females at twelve months. Conclusion: As a result fertility parameters of sport horses maintained at the institution could be said to be in good level. The management, feeding and vaccination programs should be improved to reduce the incidence of disease.

Factors effecting live weight, body measurements, disease incidence, livability and fertility of Sport horses: II. Live weight and body measurements in foals

Deniz Taşkın, Serdar Koçak — 2013-03-01

Aim: Aim of this research was to determine factors effecting live weight and body measurements in sport and friesian foals at Gemlik Military Veterinary School. Materials and Methods: The material of this study includes 118 head sport and Friesian foals in the years 2002-2009. Results: Values of live weight and body measurements of foals at different levels of the year and effect of gender was significant. Conclusion: Growth of foals maintained at the institution could be said to be in good level. High live weight and body measurements were determined in born foals between 2006 and 2009

Usefulness of a microsatellite test panel for cattle parentage testing in Turkey

2013-03-01

Aim: The objective of this study was to test usefulness a microsatellite test panel for parentage analysis in widely reared cattle breeds in Turkey. Materials and Methods: In this study, genomic DNAs were used from 148 cattle. A total of eleven bovine microsatellite loci were selected from a list suggested by ISAG and FAO MoDAD. Genomic DNA samples were amplified by polymerase chain reaction (PCR). Alleles were determined by fragment analysis after capillary electrophoresis. General population parameters including allel numbers (Na), observed (Ho) and expected heterozygosities (He), deviation from Hardy-Weinberg Equilibrium (HWE) and power of exclusion (PE) at each microsatellite locus were calculated. Results: A total of 150 different alleles were determined ranging from 6 to 17 at each locus. The Ho and He were ranged from 0.435 to 0.884 and 0.632 to 0.887, respectively. Locus based PE-1 and PE-2 were varied as 0.239-0.630 and 0.419-0.774 and a total PE-2 value was calculated as 0.999. Conclusions: It was concluded that the test panel seems to be applied in cattle identification efforts; however, there is need for increasing the number of the locus in the panel and development of efficient multiplex systems.

Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations

2013-03-01

Aim: The objective of the present study was identification of the chromosomal regions responsible for egg yield and body weight at different age periods in a Denizli and White Leghorn F2 population. Materials and Methods: An experimental F2 population was constructed by crossing Denizli and White Leghorn breeds and the yields of the animals were recorded. In chromosomal scanning trials, a total of 113 microsatellite markers, suitable for use in quantitative trait locus (QTL) gene mapping, were amplified by the polymerase chain reaction (PCR) in F0, F1 and F2 animals. Results: Data obtained in the present study demonstrated that QTL regions associated with body weight at different age periods were located on chromosome 1 (GGA1), GGA2 and GGA4. It was determined that, two different QTL regions affecting egg yield existed, on GGA8 and the sex chromosome (GGAZ). Three different QTL regions located on the chromosomes GGA2, GGA4 and GGAZ were associated with egg weight. Conclusion: There is a need for narrowing these QTL regions by typing new markers in these intervals and for identifying genes that have affect on these economically important traits.

Comparison of a new chromogenic medium with standard media for isolation and identification of Bacillus cereus

Anita Tewari, Suresh Prasad Singh, Rashmi Singh, Deepak Kumar — 2013-03-01

Aim: The aim of this study was to compare a new chromogenic plating media HiCrome Bacillus cereus agar (HIMEDIA®) against two standard selective plating media (PEMBA and MYP), recommended for isolation, identification and enumeration of Bacillus cereus. Material and Method: Twenty nine B. cereus PCR confirmed isolates of meat origin were used for evaluation of the three media. All the isolates used in this study were already characterized during research work. Results: The proportion of isolates with typical colonies was highest in MYP with 27 colonies out of 29, while HiCrome BCA and PEMBA both showed only 25 typical colonies. Isolates with weak reaction were found on all three plating media but the weak reactions were shown by 4, 2 and 4 isolates on PEMBA, MYP and HiCrome BCA, respectively, thus they were more for PEMBA and HiCrome BCA as compared to MYP media. Conclusions: Our survey showed that the MYP media was better than PEMBA and HiCrome Bacillus cereus agar for isolation and identification of B. cereus. Chromogenic media did not represent a very good alternative to the conventional standard media for diagnostic of B. cereus but it was found superior than standard plating media for enumeration of the bacteria.

Comparative efficacy of ivermectin plus clorsulon and nitroxynil against naturally infected cattle

2013-03-01

This study was conducted to determine a suitable injectable preparation against to helminthes in cattle. Fifty six cattle were divided to three groups. The first (n: 27), second (n: 16) and third (n: 14) group were treated with the drug containing ivermectin+clorsulon, nitroxynil and combination of both, respectively. In all three groups, the drugs showed 100% efficacy against Fasciola sp. In the first group, ova of Paramphistomum sp. were present in all post treatment observation and the drug showed 80% efficacy during the last observation. The ova of strongyles were absent at 60 days post treatment observation. In the second group, 100% efficacy of nitroxynil was not observed against Paramphistomum sp. and strongyles. In the third group, combined drug showed 100% efficacy against strongyles from 30 days post treatment observation up to last observation. The ova of Paramphistomum sp. were absent only at 60 days post treatment observation. The efficacy of combined drugs treatment was significantly (p<0.05) higher than ivermectin plus clorsulon treatment against Paramphistomum sp. Combination of ivermectin, clorsulon and nitroxynil can successfully be used in the control programs.

Frequency of infectious bovine rhinotracheitis in cows with repeat breeder breeding in Ergani district of Diyarbakir

Sidal Alparslan, Ali Rişvanlı — 2013-03-01

In this study, prevalence of Infectious Bovine Rhinotracheitis (IBR) was investigated from cows with repeat breeder in Ergani district of Diyarbakir province. Blood samples were collected 3-10 of different ages ranging from 108 cows. The presences of IBR antibodies in the samples were examined using Enzyme Linked Immunosorbent Assay (ELISA) kits. The results of the ELISA indicated that 52 (48.15%) of 108 animals were seropositive and other (51.85%) were seronegative. Although there are not difference between the groups in the distribution of results according to breed, according to age, IBR seropositivity from 7 years (86.67%) and older animals group were found to be higher (p<0.01). As a result, IBR seropositivity from cows with repeat breeder is high and, this ratio was more increased the older animals. Therefore, wing was concluded that the necessary measures taken against IBR for the cows in the region improve the efficiency of progeny.

Spontaneous lymphoma in a Golden hamster (Mesocricetus auratus)

Reza Kheirandish, Baharak Akhtardanesh, Nasrin Askari — 2013-03-01

In this clinical report, occurrence of a spontaneous lymphoma was discussed in a Golden hamster. Male hamster was referred to the veterinary hospital with a history of anorexia, diarrhea, prominent swelling of cervical lymph nodes and progressive weight loss since two weeks ago. During physical examination generalized lymphadenomegaly and severe dehydration was seen. Intraperitoneal dextrose and hydrocortisone was injected but the patient died. In necropsy, significant enlargements of all superficial lymph nodes were observed but the mesenteric lymph nodes, spleen, and liver were seemed normal. Dissected lymph nodes showed necrotized center. Histopathologic examination revealed lymphoid cells containing large vesicular nuclei with evident nucleoli which showed moderate mitotic index. On the basis of these findings, a definitive diagnosis of non-Hodgkin diffuse large B-ceIl lymphoma was made. Experimentally induced lymphomas have been reported in laboratory animals; however, spontaneously occurring lymphomas have been infrequently described in hamsters. This clinical report describes the clinical and histopathological aspects of a spontaneous lymphoma.

Disseminated metastatic transmissible venereal tumour in a bitch

2013-03-01

The material of this case is a 4-years-old Siberian Husky bitch with 3 months of continuous postpartum bloody vaginal discharge complaints. There were subcutaneous masses in various sizes in many points, vaginal bloody discharge, edematous vulva with fragile and easily bleeding on touch, cauliflower looking tumoral masses as noticed during physical examination. Typical transmissible venereal tumor (TVT) cells, shaped round, ovoid or polyhedral with eosinophylic vacuole, thin cytoplasm, round hyperchromatic nucleus and nucleolus were observed on cytological examination of smears prepared from tumoral masses in vagina. There were changes in echogenities of liver, spleen and left kidney, hepatomegaly, spleenomegaly and besides many mass formations in liver and one in spleen on ultrasonographic examinations. Disseminated metastasized TVT was diagnosed in the bitch depending on clinical, laboratory, radiographic and ultrasonographic examinations. Prognosis of the case was bad thus the patient was euthanized. Metastasized masses were observed during necropsy in skin, subcutaneous and around the udders, on the last rib, in abdomen, lung, liver and spleen. It was observed that TVT case, which is benign usually and metastatic rarely, turned malign and metastasized to skin, subcutaneous tissue, udders, liver, spleen and lung. In conclusion, a careful systemic examination and inquiring the presence of metastasis should be considered in dogs with TVT.