Seven-month-old male cat which had been applied an urethral catheterization in a private vet clinic was referred with anemnesis of dysuria to the Animal Hospital, University of Selcuk, Konya-Turkey. In this report, diagnostic and therapeutic approaches of iatrogenic urethral perforation which was defined on the basis of clinical, laboratory, radiographic and ultrasound findings in a male cat were evaluated. In the physical examination of the cat suffering from loss of apetite, dripping bloody urination and dysuria, dehydration and bladder distention were observed. The catheterization was performed to evaluate urinary outflow in our clinics, but urine output was not achieved. In the ultrasound examination, it was observed that the urinary catheter was located in ventral abdominal area and the thickening of bladder wall and also the bladder was observed as empty. Urinary catheter was viewed in ventral abdominal area in latero-lateral direct radiographic examination. In conclusion, it was decided to do experimental laparotomy due to pre-diagnosis of iatrogenic urethral perforation. Severe urethral injury and rupture were determined in penil urethral area, and urinary diversion was achieved by tube application from bladder to abdominal wall. After operation, the urinary output via tube was seen.