2017, Cilt 33, Sayı 1, Sayfa(lar) 040-045
The mismatched isolation of Brucella strains from nomic hosts
Özgür Çelebi1, Fatih Büyük1, Keti Sidamonidze2, Eka Zhgenti2, Doğan Akça3, Mitat Şahin1
1Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey
2Department of Virology, Molecular Biology and Genome Research, R.G. Lugar Center for Public Health Research, National Center for Disease Control and Public Health, Tbilisi, Georgia
3Kars Health School, University of Kafkas, Kars, Turkey
Keywords: Brucella, field strains, cattle, sheep, molecular characterization

Aim: A small part of field Brucella strain collection of Kafkas University was evaluated by different methods in this study.

Materials and Methods: The strains were originated from cattle and sheep which had a history of abortion. Thirty field Brucella strains (16 were originated from sheep and 14 were from cattle) were randomly selected and included in the study. In addition of standardized phenotypical characterization, genus specific real-time PCR and species specific Bruce-Ladder PCR were conducted for molecular typing.

Results: Out of 16 sheep and 14 cattle samples 15 and 13 were identified as B. melitensis and B. abortus, respectively. Interestingly one strain was characterized as B. abortus isolated from aborted sheep foetus and the other as B. melitensis from aborted cattle milk sample. Furthermore one strain from aborted sheep foetus was unable to be confirmed as Brucella spp. by genotypically (neither by real-time PCR nor Bruce-Ladder PCR) though all phenotypic features were similar to Brucella genus and kept under strict conditions for future characterization on suspicion of Ochrobactrum spp.

Conclusions: These report alleges that Brucella crossinfection among certain hosts ceased to be extraordinary and gained an usual face over time. These kind of efforts would help in further understanding the evolution, host specificity and pathogenicity of the genus Brucella.