2017, Cilt 33, Sayı 3, Sayfa(lar) 190-194
Investigation of West Nile virus infection in brown bears (ursus arctos) in Turkey
Yakup Yıldırım1, Fethiye Çöven2, Erdoğan Uzlu3, Volkan Yılmaz4, Emrah Çoban5, Ayşegül Çoban5, Çağan Hakkı Şekercioğlu6
1Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Viroloji Anabilim Dalı, Burdur
2Bornova Veteriner Konrol Enstitüsü, İzmir
3Kafkas Üniversitesi Veteriner Fakültesi İç Hastalıkları Anabilim Dalı, Kars
4Kafkas Üniversitesi Veteriner Fakültesi İç Hastalıkları Anabilim Dalı, Kars
5Kuzey Doğa Derneği, Iğdır
6Utah Üniversitesi Biyoloji Bölümü, Salt Lake City
Keywords: Brown bears, ELISA, RT-PCR, West Nile virus
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Aim: This study is a serological and virological examination of the West Nile Virus (WNV) in free-ranging brown bears (Ursus arctos) in Sarikamis district of Kars province of Turkey.

Materials and Methods: For this purpose, blood samples from 11 brown bears were tested for antibodies against WNV using a commercial competitive enzyme-linked immunosorbent assay (C-ELISA). Reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of West Nile Virus nucleic acid.

Results: Seropositivity rate was detected to be zero % and West Nile virus nucleic acid was not found in samples.

Conclusions: The significance of WNV infection in brown bears and other free-ranging mammals in the Turkey is unknown at this time and will require further investigation. This is the first report of WNV infection in brown bears in Turkey.