2018, Cilt 34, Sayı 1, Sayfa(lar) 001-006
Effect of thymoquinone on proliferation of bovine peripheral blood mononuclear cells
Uçkun Sait Uçan, Zafer Sayın, Aslı Sakmanoğlu, Ali Uslu
Selçuk Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji AD, 42250, Konya, Türkiye
Keywords: Thymoquinone, phenyl boronic acid, cattle, mononuclear cells, proliferation

Aim: Thymoquinone (Tq) is the most important component of Nigella sativa and has positive biological effects on various cells of the mammals. At present study, a possible effect of Tq on the large ruminant immune system was investigated by in vitro assays.

Materials and Methods: Thus, the effects of different Tq concentrations on both survival and proliferation of bovine peripheral blood mononuclear cells were investigated by cell culture and ELISA methods in the presence or absence of two mitogens (Con A and PWM). In addition, phenyl boronic acid (PBA) was evaluated similarly.

Results: Tq showed a blastogenic effect on peripheral mononuclear cells of cows when Tq was added to culture medium at a final concentration of 50 μg/mL. Following 5 days of proliferation of cells with 10⁷ cell/mL density in presence of 50 μg/mL Tq caused 0.485 ± 0.06 OD degree of blastogenesis (P <0.01). The mitogenic responses for Con A and PWM were 0,399 ± 0,084 and 0,397 ± 0,049, respectively. The combination of Tq with either of both mitogens (Con A or PWM) did not lead to a further increase in the proliferation. Mechanism of Tq's mitogenic effect on bovine immune cells is completely in dark at present. Additionally, PBA is present in cell culture medium at a final concentration of 2.5 μg/mL a significant degree of proliferation was measured (0,425±0,058) (P<0,01).

Conclusions: These results support our hypothesis that Tq would be a candidate for being a component of a novel adjuvant or even a promising immunostimulant for therapeutic purposes in future. Moreover, PBA is concluded as another potential compound for mitogenesis of bovine immune cells.