2018, Cilt 34, Sayı 3, Sayfa(lar) 178-184
Phenotypic and molecular identification of extended-spectrum beta-lactamases in Escherichia coli in healthy broilers at the west region of Iran
Sina Sheikhi1, Elham Ahmadi2
1Graduate of Faculty of Veterinary Medicine, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2Department of Pathobiology, Faculty of Veterinary Medicine, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
Keywords: Antibiotic resistance, ESBL-producing Escherichia coli, Iran

Aim: The presence of fecal carriers of extended-spectrum beta- lactamase (ESBL) genes in poultry is worrisome because it is likely to spread to a human being. This study was proposed to estimate the frequency of ESBLs carriers in Escherichia coli isolated from healthy broiler’s gut in west of Iran.

Materials and Methods: A total of 300 fecal samples were collected from apparently healthy broiler. E. coli strains were isolated, confirmed molecularly and studied using disk combination method, antimicrobial susceptibility test and ESBL-detection polymerase chain reactions (PCR).

Results: Two hundred and eighty five strains of E. coli were isolated biochemically and all were confirmed in a speciesspecific PCR reaction. Antimicrobial susceptibility test on 156 ESBL-producing E. coli identified by phenotypic method revealed that 100 % of them were resistant to at least one antibiotic, while all were sensitive to imipenem. The prevalence of blaTEM, blaCTX-M, and blaSHV genes was 52.63%, 38.59%, and 30.17% respectively. The simultaneous existence of several ESBL genes was identified in 52.61% of the isolates.

Conclusions: The vast prevalence of ESBL carriers with multiple resistance in poultry is a threat to public health. Consistent monitoring and proper use of antibiotics in the region’s poultry industry should be applied.