2020, Cilt 36, Sayı 2, Sayfa(lar) 096-101
The investigation of Israil acute bee paralysis virus, sacbrood virus, kashmir bee virus and chronic bee paralysis virus in honeybees (Apis mellifera)
Abdurrahman Anıl Çağırgan1, Yakup Yıldırım2, Ayşegül Usta2
1Bornova Veterinary Control Institute, Department of Virology, Bornova, Izmir, Turkey
2Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Virology, Burdur, Turkey
Keywords: Israil acute bee paralysis virus, kashmir bee virus, chronic bee paralysis virus, Reverz Transcriptase-Polymerase Chain Reaction (RT-PCR), sacbrood virus
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Aim: This study aims to investigate the presence and prevalence of Israel acute bee paralysis virus (IAPV), Sacbrood virus (SBV), Kashmir bee virus (KBV) and Chronic bee paralysis virus (CBPV) by Reverz Transcriptase-Polymerase Chain Reaction (RT-PCR) method in apiaries around Burdur region.

Materials and Methods: During the study, 30 beehives from 15 apiaries were selected randomly and sampling procedures were carried out. 30 adult bees were collected from each of the sampled hives to form a pool. After the samples were homogenized, total nucleic acid extraction was performed and then viral nucleic acid was searched by RT-PCR method using specific primers.

Results: In this study, in consequence of analyzing 30 samples from 15 apiaries by RT-PCR method, SBV was detected in 10 samples obtained from 5 apiaries (33.3%) and CBPV was found in 4 samples from 2 apiaries (13.3.%). IAPV and KBV were not detected. As a result of evaluations, 4 samples from 2 apiaries were considered to have been infected with both SBV and CBPV, which means a mixed infection occurred in these hives.

Conclusion: It is clear that the so-called diseases were regarded important in terms of beekeeping in not only Burdur region but also in the other regions of Turkey and serious precautions should be taken to prevent infections. Struggling with bee parasites that play the key role in transmission viral agents and enhancing the immune resistance of bees and obeying the rules of hygiene and sanitation should not be ignored in protecting bee health. Besides, the future studies on honeybee viruses should be planned to include wider areas and populations and to reveal viral phylogenies.