The present study aimed at examining the presence of antibodies against enzootic bovine leukemia virus by enzyme-linked immunosorbent assay (ELISA) method in blood samples collected from 345 female cattle in Elazig and Malatya and their vicinity. As a result of the study, 9 out of 345 animals (2.6%) were found seropositive. When compared lo the seropositivity rale determined in previous studies in our region, this rate was found high. The fact that the studied animals were female makes this rale of seropositivity more important epidemiologically.