In this report, hepatocellular carcinoma in a 5-years-old female Van cat was described. Grossly, grayish-white nodular masses o( 0,1 -6 cm in diameters were found in the liver, spleen, lung, kidneys, abdomen and under the skin. Microscopically, lumor cells were arranged as solid, cord-like and trabecular pattern. Neoplastic cells usually had polygonal and vesicular nuclei and had multiple mitotic figures. Immunohistochemically some tumor markers such as alpha-fetoprotein (AFP), cytokeratin, smooth muscle actin (SMA), and factor Vlll-related antigen (FVIII-Rag) was also studied, and AFP and SMA were found to be positive.