This study was conducted with healthy, fertile 10 Holstain dairy cows that have same breeding, feeding and environmental factors in Konya Animal Central Research Institute. Blood sera, heparinized and EDTA plasma were obtained from V. Jugularis blood samples; alkaline phosphatase(AP), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT). albumin, cholesterol, magnesium (Mg), calcium (Ca), inorganic phosphor (Pi), copper (Cu) and zinc (Zn) values were dotermined immediately with a serious of the samples and this parameters were re-peadly measured after stored 100 days in - 20 °C, Cu, Zn values were measured by atomic absorbsiyon spectrophotometer (Buck Scientific modem 200A). Other analyses were made by spectrophotometer (Shimadzu UV-2100). In serum, EDTA and heparinized plasma treatments for 0. days Ihere were found significant differences between groups as albumin (P<0.01), P (P<0,001), Mg (1X3.05).Cholesterol (P>0.05), AP (P<0.001), ALT (P>0.05) GGT (P>0,05), Cu (P<0.01), Zn (P<0.001), Ca (P<0.01). In addition this differences between groups for 100. days were found to be P<0.001; P<0.001; P<0.001; P>0.05; P<0.001; P>0.05: P>0.05; P>0.05; P<0.001; P<0.001 respectively. For the all parameters dillerences between 0. and 100. days were revised in treatment groups and obtained various statistically significant results.