The aim of the study was to evaluate the bacteria levels of non-sprayed and sprayed eggs with a water-based disinfectant from a layer unit of the Veterinary Faculty Farm of SU. A total number of 80 eggs were used. Eggs with Poto- Clean® treated or not treated were examined by aerobic and Gram negative bacterial contamination using conventional culture techniques. A significant decreases in the counts of aerobic bacteria and Gram negative bacteria were found (p<0.001). Thus, this sanitizer showed a general ability to reduce bacteria on egg shells to a negligible number. The disinfectant tested is highly promising for such purpose.