The aim of this study were to determine the relations of seminal fructose, corrected seminal fructose, fruc-tolysis index, seminal plasma Zn and blood plasma Zn, blood plasma testosterone levels with semen quality in healthy rams. The study was carried out in 23 fertile and healthy rams of which was 5 Akkaraman, 5 Merinos, 5 Awassie, 5 Corriedale and 3 German black face breeds, aged between 2-3 years old. The semen samples of each ram were collected two consecutive ejaculates by artificial vagina and blood samples 4 times every 3 days. Mean spermatozoon individual motility (%) for Akkaraman, Merinos, Awassie, Corriedale and German black face breeds were 70.5±1.9, 75.0±1.67, 73.0±1.66, 76.5±1.98 and 68.1±1.88 (%), respectively. Mean sperm concentration (x10⁶/mm³) of the breeds were 3.16±0.26, 2.70±0.25, 2.93±0.30, 2.42±0.33 and 1.66±0.23 respectively. Mean fructose (mg/dl), corrected fructose (mgfmlxlog.sperm concentration/ml), fructolysis index (mg/10⁹ sperm/1 hour,37 ºC), seminal plasma Zn (µg/dl), blood plasma Zn (µg/dl) and testosterone (ng/ml) levels were 663.10±100.5, 63.02±9.64, 1.68±0.39, 612.0±73.2, 81.1±8.3 and 5.62±0.74 in Akkaraman rams; 395.30±42.27, 37.17±3.94, 0.99±0.27, 488.6±76.4, 7S.6±8.3 and 3.66±0.64 in Merinos rams; 522.10±82.81, 49.36±7.88, 1.39±0.34, 590.3±98.8, 70.6±11.1 and 4.89±1.08 in Awassie rams; 904.70±204.6, 83.96±18.68, 2.80±0.86, 378.8±99.0, 57.2±7.5 and 5.28±0.84 in Corriedale rams; and 470. 13±98.87, 43.37±8.98, 1.91±1.10, 584.10±145. 86.90±9.8 and 5.62±1.72 in German black face rams, respectively. The positive correlation was observed between the seminal fructose and the fructolysis index l (P<0.001) in all breeds. In conclusion, it is indicated that fructolysis index which is a important criteria for determining sperm quality correlated with sperm fructose concentrations; therefore determining fructose level will be very useful in spermatologic trials.