In the study the effects of aflatoxin added to ration and used glucomannan to prevent allatoxin absorption on some biochemical parameters and antioxidants were studied in 40 New Zealand rabbits. The rabbits were separated to four groups as control (K), glucomannan (G), glucomannan + aflatoxin (AG) and aflatoxin (A). At the end of the ten weeks the MDA levels increased, GSH and SOD levels decreased (p<0,05) in A group compared with control group levels. Cholesterol, glucose, albumin and total protein lovels also decreased, AST and ALT levels increased in A group compared with control group. In the G group the former parameters were not affected by glucomannan application alone. On the other hand in the AG group MDA, GSH, SOD, cholesterol, glucose, albumin and total protein levels were higher, while AST and ALT levels were lower then control levels although these differences were not significant. In conclusion, the results determined in this study, might be important to demonstrate the effects of aflatoxicosis on some biochemical and antioxidant parameters at least applied dose of allatoxin and glucomannan.