Efficiency of the real-time linear array ultrasonography to observe nonpregnant uterus in mature and sexually healthy bitches were evaluated. A total of 29 bitches of various breds, 24 intravital, 3 postvital and 2 males, were used. In the preliminary experiments, postvital or intravital, ultrasonographic examinations of uterus were performed either on in vitro uterus pieces or following laparatomy, cathether applications and fluid infusion into vagina. Male dogs were used as control. In the main part of the study (n=20) ultrasonographic images of different parts of uterus were obtained when bitches were laying on one side or at up side down position by applying the probe on the pelvic area. Cornu uteri could not be screened ultrasonographically in 18 cases (% 90) out of 20. In two cases, both of the uterine horn with different echogenicity from each other was detected. Cervix and corpus uteri were viewed in all cases (%100). In one of the two bitches with ultrasonographically visible uterine horn, cornu uteri was completely hyperechogenic. However, in the second case uterine horn were noticed with hyperechogenic dorsal and ventral wall and hypoechogenic inner part. Cervix uteri was observed with hyperechogenic dorsal and ventral wall, comparatively hypoechogenic inner part and anechogenic lumen. As a result, detection of the sonomorphological features of mature and nonpregnant uterus may be helpful to diagnose pregnancy and uterine pathology which are characterized by the altered uterine echotexture in bitch.