2010, Cilt 26, Sayı 1, Sayfa(lar) 033-038
Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis
Musa Karaman1, Hasan Özen1, Serpil Dağ Erginsoy1, Mitat Şahin2
1Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji AD, Kars, Türkiye
2Kafkas Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji AD, Paşaçayırı, Kars, Türkiye
Keywords: Leptospirosis, ubiquitin, 8-hydroxyguanosine, pathogenesis, cattle
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Aim: This study was performed to investigate the role of ubiquitin and 8-hydroxyguanosine (8-OHG) expression in liver and kidney tissue damage in Leptospira-infected cattle.

Material and Method: Localization of these molecules was determined by indirect immunoperoxydase method using specific polyclonal antibodies in Leptospira positive (n=7) and healthy control animals (n=7).

Results: A cytoplasmic immunostaining in the hepatocytes and kidney tubule epithelia against ubiquitin antibody was detected in Leptospira-infected animals. Immunostaining against 8-OHG antibody was seen intracytoplasmic and/or intranuclear in the hepatocytes, while it was mostly intracytoplasmic in kidney tubule epithelia.

Conclusion: As ubiquitin and 8-OHG were shown to play roles in pathogenesis of Leptospirosis, further detailed studies to investigate and to wholly understand the mode of mechanisms of these molecules are suggested.