2010, Cilt 26, Sayı 2, Sayfa(lar) 109-112
Serodiagnosis of Brucella canis infection in dogs by a dipstick enzyme immunoassay
Uckun Sait Ucan1, Zeki Aras1, Ahmet Semacan2
1Department of Microbiology, Faculty of Veterinary Medicine, Selcuk University, Campus, Konya, Turkey
2Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Campus, Konya, Turkey
Keywords: B.canis, EIA, iELISA, dog
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Aim: A rapid and reliable test is needed by the veterinary practitioners although the canine brucellosis can be diagnosed by bacteriological, molecular and serological methods. By this study, developing a dipstick EIA for a rapid serodiagnosing the infection was aimed.

Materials and Methods: The test was compared with Rapid Agglutination Test iELISA.

Results: Sensitivity and specificity of the test were 82% and 95%, respectively when iELISA was evaluated as gold standard.

Conclusion: Since the test has a good degree of specifity it is recommended as a screening test in the field.