2011, Cilt 27, Sayı 2, Sayfa(lar) 087-092
Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semen
Nuri Başpınar1, Kenan Çoyan2, Mustafa Numan Bucak2, Ali Doğan Ömür2, Mehmet Bozkurt Ataman2, Pınar Peker Akalın3, Şükrü Güngör2, Caner Öztürk2
1Selçuk Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kampüs, Konya, Türkiye
2Selçuk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Kampüs, Konya, Türkiye
3Tarım ve Köyişleri Bakanlığı, Merkez Veteriner Kontrol ve Araştırma Enstitüsü, Ankara, Türkiye
Keywords: Ram semen, freezing-thawing, lipoic acid
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Aim: The aim of this study was to investigate the effects of different doses of lipoic acid added into dilution extender, following equilibration and freeze-thawing of ram semen on spermatologic and biochemical parameters.

Materials and Methods: The ejaculates collected from 6 mature Konya Merino rams were used in the research. Ejaculates containing approximately 400x106 spermatozoa were diluted at 37 ºC with the Tris-based extender containing lipoic acid at the doses of 1, 2, 4 mM and no additive (control), and then, they were frozen.

Results: After equilibration, all doses of lipoic acid did not affect the sperm parameters whereas lipoic acid 1 mM (74.3%) ameliorated motility compared to both control (62.5%) and lipoic acid 4 mM (65.0%) (p<0.001) groups after freeze-thawing. Lipoic acid did not affect HOS test and acrosome integrity of spermatozoa following freeze-thaw-ing. Also, the supplementation of lipoic acid at 1, 2 and 4 mM doses did not provide any significant effect on the levels ofbiochemical parameters (p>0.05).

Conclusion: Addition of 1 mM lipoic acid to ram semen increases the motility after freeze-thawing process. However, dose treatments of lipoic acid in different extenders and animal species-breeds are required to be performed.