2011, Cilt 27, Sayı 3, Sayfa(lar) 139-143
Investigation of carbonic anhidrase 1 (CA 1) and malic enzyme (ME) in Acıpayam sheep by using starch gel electrophoresis
Ümmühan Özdemir1, Mehmet Nizamlıoğlu2
1İbrahim Hakkı Konyalı İlköğretim Okulu Fen ve Teknoloji Öğretmeni, Meram, Türkiye
2Selçuk Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kampus, Konya, Türkiye
Keywords: Acipayam sheep, electrophoresis, genetic, polymorphism

Aim: For the determination of genetic structure of Acipayam Sheep, two enzyme loci were studied by using starch gel electrophoresis; carbonic anhidrase 1 (CA 1) and malic enzyme (ME). Materials and Methods: In this study, blood samples were taken from 50 sheeps in "Denizli Acıpayam State Farm". Hemolysates were examined by electrophoresis. Tris-ED-TA-Borat pH:8.6 buffers were used for both of the enzymes. In staining after electrophoresis, MUA, acetone, agar noble, phosphate buffer pH:6.5 was used for carbonic anhidrase 1. NADP, MTT, PMS, DL Malic acid, MgCl2, NaOH, agar noble, Tris-HCl pH:8.0 buffer was used for malic enzyme. Bands were observed in ultraviolet lamps for CA 1 and appropriate light condition for ME. Results: No polymorphism was observed for the CA 1 enzyme. All sheep were found to be homozygote for the same allele. For ME loci, population were observed polymorphism, having F, FS and S bands, the difference between F and S bands in ME loci were unimportant (p>0.05) and allels were determined in the Hardy-Weinberg equilibrium. Conclusion: In this study, ME polymorphism was observed but no polymorphism was determined for CA 1 locus. It was concluded that it is necessary to investigate other enzymes in terms of polymorphism.