In Burdur province, the infection of Bovine Viral Diarrhea Virus (BVDV) was investigated clinically, serologically and virologically in a dairy herd having the problems of the Repeat Breeding. For this purpose, serum and leucocyte samples from 25 cattle and 8 calves were collected. Serum samples for the presence of antibody (Ab) and leucocyte samples for the presence of antigen (Ag) were examined by ELISA. In the study, the presence of BVDV-Ab in serum samples, obtained from 7 calves and the presence of BVDV-Ag in leucocyte samples obtained from these calves were not detected. The presence of Ab and Ag in serum and leucocyte samples belonging to the dams of these calves were not detected. But, the presence of Ag in leucocyte samples of the same animal was detected while the presence of Ab in sera sample ofa calfwas not detected. The dam of this calfwas negative serologically and was positive virologically. The leucocyte samples obtained from this cow and its calf after 45 days were retested .They were described as persistent infected (PI) animals (6.06%). However, the presence of Ag in leucocyte samples obtained again from cattle found 7 BVDV (Ab-/Ag+) and 4 BVDV (Ab+/Ag+) after 45 days was detected and these animals were also decided to be PI.