In this study, arteriel vascularization of septum interventricular was invastigated macroscopically. For this purpose, it was used ten adult Kangal dogs in different sex and age. Supplying of septum interventriculare was essentially provided by ramus septalis. It was defined that septum interventricular also supplyed by vessels originating from ramus interventricularis paraconalis and ramus interventricularis subsinuosus in peripheral.