1992, Cilt 8, Sayı 2, Sayfa(lar) 060-063
Determination of N-acetyl β-D glucosaminidase enzyme activity in bovine milk for the early diagnosis of subclinical mastitis
Mehmet Nizamlıoğlu1, Leyla Kalaycıoğlu2, Dursun Ali Dinç3, Osman Erganiş4, Firuze Özeren5
1Doç. Dr., S. Ü. Veteriner Fakültesi, Biyokimya Anabilim Dalı, Konya
2Prof. Dr., S. Ü. Vet. Fak., Biyokimya Anabilim Dalı, Konya
3Doç. Dr., S. Ü. Vet. Fak., Doğum ve Reprodüksiyon hastalıkları Anabilim Dalı, Konya
4Doç. Dr., S. Ü. Vet. Fak., Mikrobiyoloji Anabilim Dalı, Konya
5Arş. Gör., S. Ü. Vet. Fak., Biyokimya Anabilim Dalı, Konya
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In this present study, N-acetyl-B-D-glucosaminidase (NAG ase) activity and microbiological investigations were carried out in milk samples for the early diagnosis of subclinical mastitis in dairy cows.

Forty Holstein cow, in 4-5 years old were used as materials.

NAG ase activity, PMN and microbiologic values, in milk samples in which CMT test performed, were compared.

NAG ase activity was found 33.08±4.62 U/L and 90.70±11.98 in healty and mastitic milk sampes respectively and differences were found statistically significant (P<0.05).

The PMN values were determined average 282655±57035 in healty milk and 1236396±216308 in mastitic milk samples.

Staphylococcus, Klebsiella pneumonia, Corynebacterium ssp agents were identified in milk samples.

As a result, it was useful to determine NAG ase activity, PMN and CMT test in milk samples for the early diagnosis of subclinical mastitis.