Materials and Methods: Antemortem examination of 5 suspected cats were evaluated. Brains were collected from the suspected cats. Direct Fluorescent Antibody Test (dFAT), Hematoxylin and Eosin (H & E) and Immunohistochemistry (IHC) histopathological diagnostic tests were applied.

Results: The dFAT test was conducted on the saliva and fresh brain impression smear of all cats, among that 4 cats showed positive for rabies virus. After death of cats, Hematoxylin & Eosin (H&E) and Immunohistochemistry (IHC) techniques were carried out. One case showed meningitis and remaining showed Negri bodies. Viral antigen depositions were observed by counting 100 cells each region of hippocampus and cerebellum by H&E and IHC. Hippocampus pyramidal cell showed 87% and cerebellum Purkinje cells showed 69% of rabies viral antigen deposition.

Conclusion: The IHC can be used as reliable diagnostic technique in addition to dFAT. IHC shows positivity in mildly infected cases and having immense value for retrospective studies. It also minimizes the risk of public health hazard during shipping of rabid positive brain samples.