2013, Cilt 29, Sayı 2, Sayfa(lar) 097-102
Detection of Bovine Viral Diarrhea Virus antibodies in blood and milk serum in dairy cattle by ELISA
Sibel Yavru1, Atilla Şimşek1, Mehmet Kale2, Oya Bulut1, Orhan Yapıcı1,3, Oğuzhan Avcı1, Irmak Dik1
1Selçuk Üniversitesi, Veteriner Fakültesi, Viroloji ABD, Konya, Türkiye
2Mehmet Akif Ersoy Üniversitesi, Veteriner Fakültesi, Viroloji ABD, Burdur, Türkiye
3Türkiye-Kırgızistan Manas Üniversitesi, Veteriner Fakültesi, Bişkek, Kırgızistan
Keywords: Blood, milk, serum, BVDV, ELISA
Abstract
Aim: The aim of this study was to detection of Bovine Viral Diarrhea Virus (BVDV) antigens and determine of antibodies against BVDV in blood and milk serum.

Materials and Methods: Totally 202 cattle blood and milk sera samples were used. Samples obtained from herds were analyzed for the detection of BVDV antigens by direct Enyzme Linked Immunosorbent Assay (ELISA) and for determine the presence of antibodies against BVDV by indirect ELISA.

Results: While 167 of blood serum (82.6%) were positive for the presence of BVDV antibodies, 160 of milk serum samples (79.2%) were positive. In this study, 148 (73.2%) both blood and milk serum samples of same cattle were positive for BVDV antibodies. Only 19 (9.4%) blood serum of cattle was detected positive for BVDV, while only 12 (5.94%) milk serum was seropositive. No positive result to be determined for BVDV antigens in any blood or milk serum samples.

Conclusion: Determined similar indirect ELISA results of blood and milk sera (P>0.05) may be suggest that milk sera can be used in determine of serological diagnosis of BVDV instead of blood sera. Further research is needed in order to achieve using blood and/or milk serum samples presence of BVDV antigens instead of leukocytes samples in routine diagnosis, even though not detect the presence of BVDV antigen in blood and milk serum samples in this study.

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