Eurasian Journal of Veterinary Sciences - 2014; 30(1)

Macroanatomical investigations on the intrahepatic ramification of the portal vein in the native donkeys

Yasin Demiraslan, Kadir Aslan, İftar Gürbüz, M. Orhun Dayan — 2014-03-01

Aim: It was aimed to determine the intrahepatic ramification and course of the portal vein in the donkeys. Materials and Methods: 6 donkeys (3-females, 3-males) were used as the material. After opening the abdominal cavity of the donkeys, whole blood was drained under general anesthesia, it was reached to liver. Portal vein was washed with saltwater 0.9%, and colored latex was injected to it. The livers, kept in the tap water for 48 hours, were dissectioned. Results: In this study, it was detected that the portal vein brought the 6-stout (mean: 5.75 mm) and 2-slim (mean: 1.57 mm) branches before it produced the ramus dexter. It was determined that the 6-stout branches progressed in the caudal, cranio-dorsal, right caudo-dorsal, right intermediate, right cranio-dorsal and right cranio-ventral directions in the right lobe of the liver. It was identified that other slim two branches spreaded in regional liver tissue. It was detected that the ramus dexter with the somewhat large diamater (mean: 11.32 mm) was divided into two branches as the right caudo-ventral and quadrate lobe directions. It was set that the ramus sinister supplied blood with 7-branches, with average thickness 4.84 mm, to the quadrate and left lobe of the liver. These branches coursed to the left cranio-dorsal, left medial, quadrate lobe (2), left intermediate, left caudo-ventral and left cranio-ventral directions. Conclusions: Although the intrahepatic ramifications of the portal vein of the horse and donkey were mostly similar to each other, some differences existed with regard to conformation of vessels.

The effect of intravenously given dexketoprofen trometamol on postoperative pain in ovariohysterectomized dogs

Zulfikar Kadir Saritas, Musa Korkmaz, Oktay Yilmaz — 2014-03-01

Aim: The aim of this study was to investigate the postoperative analgesic effect of dexketoprofen trometamol in dogs subjected to ovariohysterectomy. Materials and Methods: Seventeen adult bitches were randomly allocated to two groups. Dexketoprofen trometamol (n=10, 1 mg/kg, IV) was administered to DEX group after recovery of anaesthesia, while those assigned to the control (C) group (n=7) were not given any analgesic after recovery of anesthesia. The ovariohysterectomy procedure was performed on all dogs by the same researcher using a median laparotomy access. Heart rate (HR), respiratory rate (RR) and body temperature were measured during the postoperative period. Pain level was assessed by two researchers 0, 1, 4 and 6 hours after surgery. A modified University of Melbourne Pain Scale (UMPS) was used to evaluate pain in both groups. Results: According to modified UMPS test, postoperative first hour pain score decreased statistically significant (P<0.05) in the DEX group. Although at 6th hour pain scale is lower in DEX group, no significant difference was (P>0.05) determined at other measurement times. Conclusions: The presence of stable vital signs of dexketoprofen administration is a promising finding. Dexketoprofen may used for postoperative pain management in dogs due to its clinical advantage shown by the pain scale.

Aspergillus species associated with dead-in-shell chick embryo in some hatcheries in Northwest Nigeria

Clara N. Kwanashie, Haruna M. Kazeem, Jarlath U. Umoh, Paul A. Abdu — 2014-03-01

Aim: The present work was carried out to determine the prevalence of Aspergillus species in dead-in-shell chick embryos. Materials and Methods: A total of three thousand dead-in-shell embryonated chicken eggs were collected from the four hatcheries over a period of six months. The content of 10 eggs were pooled after decontamination of the egg surface and swab of pooled contents inoculated onto the entire surface of Sabourauds Dextrose Agar (SDA) and Corn Meal Agar (CMA) slants and growths. Results: Out of the 300 groups of pooled eggs a total of 122 (40.67%) isolates of fungi belonging to 4 species of the Genera Aspergillusviz A. fumigatus, A. niger, A. flavus and A. terreus made up48.40% (59), 22.10% (27), 22.30% (26) and 5.70% (70) of the 122 Aspergillus respectively were isolated. Conclusions: The presence of these Aspergillus species indicates that they may have been primary or secondary contributors to the embryonic mortality. Decontamination of hatcheries at regular intervals is recommended for control of these fungi.

Identification and antibiotic susceptibility of coagulase negative staphylococci isolated from dairy cows with subclinical mastitis

H. Hüseyin Hadimli, Zafer Sayın, Osman Erganiş, Kürşat Kav, Aslı Sakmanoğlu — 2014-03-01

Aim: This study was aimed to identify of coagulase negative staphylococci (CNS) isolated from bovine milk samples with subclinical mastitis and to determine susceptibility of microorganisms to antibiotics. Materials and Methods: Totally, 286 CNS isolates from milk samples were identified according to colony morphology, haemolysis and biochemical properties. Results: Out of isolates, 40 (14.38%) were identified as S. simulans, 31 (11.15%) S. epidermidis, 54 (19.42%) S. chromogenes, 31 (11.15%) S. xylosus, 22 (7.91%) S. caprae, 19 (6.83%) S. warneri, 18 (6.47%) S. haemolyticus, 13 (4.67%) S. cheiferi subsp. coagulans, 10 (3.59%) S. cohneii subsp. urealyticus, 10 (3.59%) S. saprophyticus, 6 (2.15%) S. gallinarum, 7 (2.51%) S. hominis, 4 (1.43%) S. hyicus, 2 (0.71%) S. cheiferi subsp. cheiferi, 9 (3.23%) S. lentus and 2 (0.71%) S. sciuri. The susceptibilities of CNS were determined to be variable to different antimicrobial agents. Conclusions: This study was shown that identification of CNS isolated from dairy cows with subclinical mastitis should be strictly performed.

Investigations on Listeria spp. in partridge (Alectoris chukar) meat

Gürkan Uçar, Nihat Telli, K. Kaan Tekinşen, A. Ezgi Telli, H. Ahu Kahraman — 2014-03-01

Aim: In this study, it was aimed that to determine the presence of Listeria spp. and especially Listeria monocytogenes in partridge meats for putting forth the importance on public health. Materials and Methods: The survey included 10 male and 10 female partridge carcasses. Samples were obtained from a private farm which performs partridge breeding. After the cutting of partridges under aseptic conditions in a private slaughterhouse, samples were brought in to the laboratory and analyzed. Food and Drug Administration (FDA) method was used for the isolation and identification. Results: Two of the (10%) samples analyzed were found to be contaminated with Listeria spp. 10 isolates collected from the contaminated samples. These samples identified as L. innocua (5, 50%), L. grayi (4, 40%) and L. welshimeri (1, 10%). Conclusions: As a result, findings showed us partridge meat may be a public health risk and preventive measures should be taken. For this purpose hygienic conditions have to be proven on breeding and slaughtering enterprises, prevent cross- contamination and kept the cold chain until consumption.

Some morphological traits of Tarsus Çatalburun breed of Turkish hunting dog

Yusuf Ziya Oğrak, Atila Yoldaş, Milivoje Urosevic, Darko Drobnjak — 2014-03-01

Aim: The present study was carried out to determine some morphological traits of Tarsus Çatalburun breed of Turkish hunting dogs under breeding condition in their homesteads, south Anatolian cities of Adana and Tarsus. Materials and Methods: Twenty-two Çatalburun dogs (12 males and 10 females) ranging in age from 2 to 6 years were used. Gender differences for all variables were tested using Student-t test. Results: This study, which used 13 measurements taken from different parts of the dogs, can be considered one of the first scientific studies about Tarsus Çatalburun dog. The means for wither height, rump height, body length, chest circumference, chest width, chest dept, front wrist circumference, back wrist circumference, head length, head circumference, head width, nozzle length and ear length were 50.78±1.02, 49.45±1.01, 54.29±0.93, 64.75±0.75, 15.75±0.24, 20.97±0.27, 11.93±0.22, 10.84±0.26, 26.95±0.29, 41.07±0.41, 11.36±0.12, 12.18±0.19 and 17.95±0.25 cm, respectively. There were significant differences (P<0.01) between the male and bitch dogs for the means of wither height, rump height, body length, chest circumference, back wrist circumference and head length. Conclusions: Further studies need to be done in order to standardize the morphological characteristics of Tarsus Çatalburun dogs along with a pedigreed and systematic breeding program.

Effects of storage length on the hatchability of ostrich (Struthio camelus) eggs

Yusuf Ziya Ograk, Ahmet Altinel — 2014-03-01

Aim: This study was carried out in order to determine the effects of storage length on the hatchability of ostrich eggs obtained from private ostrich farms. Materials and Methods: The eggs, which were collected from ostriches in one season, lasting from March to September, were numbered, weighed, recorded with laying date and stored for 1-15 days. On the 14^th day of incubation, infertile eggs were determined and removed from incubation. On the 38^th day of incubation, eggs were weighed for the second time, and following weight losses measurement, the eggs were transferred to the hatching machine. The hatched chicks were weighed and hatchability of fertile egg, hatchability of egg set and chick weight results were determined. Results: Average incubation period was 43.58 days. In terms of incubation period, differences between groups that were classified according to storage periods (short, medium and long) were significant (P<0.05). Average egg weight loss of during incubation was 13.70%. Mean hatchabilities of egg set and fertile egg were 57.90% and 75.00%, respectively and differences between the groups of storage period were statistically significant (P<0.05). Conclusions: The storage period more than ten days has the potential to negatively affect the incubation results of ostrich eggs. So, it could be recommended that for storing ostrich eggs, 10 days of storage shouldn't be exceeded.

First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines

2014-03-01

Coxiella species are known to be potentially pathogenic tickborne organisms. This study was aimed to investigate the presence of Coxiella sp. in Rhipicephalus sanguineus ticks in Cebu, Philippines by molecular based techniques. A total of 164 ticks were collected from 36 dogs and analyzed by a Nested Polymerase Chain Reaction (PCR) for the amplification of partial 16S ribosomal RNA gene region. Nine ticks (5.5%) obtained from 5 dogs were found positive. Sequencing revealed partial DNA fragments of Coxiella spp. which were 96.3% and 98.1-100% identical to Coxiella burnetii and Coxiella sp. endosymbiont, respectively. The detected DNA fragments also shared 98.3-100% identities with each other. This study is the first report on the existence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines.

Natural ovine pulmonary adenocarcinoma in an Egyptian sheep farm

Khaled Radad, Sary Khalil — 2014-03-01

Ovine pulmonary adenocarcinoma is a naturally occurring retrovirus-induced contagious lung neoplasm in sheep. The current report aims to describe clinical and histopathological characteristics of the disease in an Egyptian sheep farm. In which, 4 dead and 5 sick young sheep were subjected to clinical, gross and histopathological examinations. For histopathological examination, tissue specimens were taken from lungs, mediastinal lymph nodes, liver, heart, kidneys and brain, fixed in 10% neutral buffered formalin and processed for light microscopy. Examined animals showed release of copious amount of mucoid fluid from their nostrils and marked loss of body weight. At necropsy, there were many solid grayish nodules exuding frothy fluid in the lungs and accumulation of mucoid fluid in the pleural cavity. Histopathological examination revealed presence of many nonencapsulated neoplastic foci originating from the alveolar epithelial lining. Neoplastic foci consisted of cuboidal and columnar cells that arranged in papillary and acinar forms. Proliferating cells appeared supported by loose connective tissue containing some fibroblasts and small blood vessels. Prominently, there was large number of swollen and ruffled macrophages in alveolar spaces adjacent to neoplastic foci. In conclusions, release of copious amount of mucoid fluid from nostrils and transformation of alveolar epithelial lining into cuboidal and columnar cells are pathognomonic for ovine pulmonary adenocarcinoma.

Cerebellar cortical abiotrophy in a Samoyed dog

Mehdi Saberi, Reza Kheirandish, Shahrzad Azizi, Elham Mohebbi — 2014-03-01

Cerebellar abiotrophies have a non-sex-linked, autosomal, recessively inherited basis in anumber of animal species, and lesions typically reflect progressive loss of Purkinje cells. In this report, ataxia was observed in a one-week-old male Samoyed puppy with no history of trauma or any other illness. Physical examination revealed uncoordinated movements, mild trunkal ataxia and intensive head tremors, so the dog had trouble eating, evacuating and difficulties in standing and walking without falling. The complete cell count, cerebrospinal fluid analysis and cerebral radiographs were normal. Treatment with diazepam, vitamin B1 and glucocorticoids had no effect on clinical signs and the dog died after a month. According to clinical signs and histopathological features, cerebellar cortical abiotrophy was diagnosed. This is the first report of a cerebellar cortical abiotrophy in a Samoyed puppy.