This study aimed to investigate the prevalence of dermatophytosis in domestic cats with skin lesions in Ankara province and to compare the effectiveness of different culture media for isolation. Skin and hair samples collected from a total of 195 cats were examined. For this purpose, a range of investigative techniques were employed, including direct microscopy, examination with Wood's lamp, cultivation, the slide culture method and the hair perforation technique. A comparative evaluation was conducted to ascertain the suitability of various media for the cultivation of dermatophytes. The prevalence of dermatophytosis was found to be 14.36% (28/195). Of the twenty-eight dermatophyte isolates, twenty-six were identified as Microsporum canis and two as Nannizzia gypsea. Moreover yeast and mycelial fungal agents such as Aspergillus spp., Alternaria spp., Bipolaris spp., Curvularia spp., Candida spp., Malassezia spp., Scopulariopsis spp. were isolated and identified. The differential diagnosis of Candida species was conducted through the implementation of gram staining, germ tube tests and Candida Chromogenic Agar passage. The results of these tests enabled the identification of two different species: Candida albicans and Candida krusei. In addition to these results, it has been determined that Sabouraud Dextrose Agar and Potato Dextrose Agar are more suitable for use in the diagnosis of dermatophytes than other culture media. As a conclusion, this study suggests that further research should be conducted to report on the current epidemiology of dermatophytosis agents, which are important for public health, and to improve the media and techniques used in diagnosis.