2018, Cilt 34, Sayı 3, Sayfa(lar) 164-170
Presence and antibiotic resistance of Salmonella spp. isolated from chicken meat and giblets consumed in Konya, Turkey
Arife Ezgi Telli1, Yusuf Biçer1, Hatice Ahu Kahraman2, Nihat Telli3, Yusuf Doğruer1
1Selcuk University Faculty of Veterinary Medicine Department of Food Hygiene and Technology Konya
2Mehmet Akif Ersoy University Faculty of Veterinary Medicine Department of Food Hygiene and Technology Burdur
3Konya Technical University, Food Technology, Konya, Türkiye
Keywords: Antibiotic resistance; chicken; S. Enteritidis; S. Typhimurium; Salmonella spp

Aim: The present study was on the detection of Salmonella spp. and two important Salmonella serotypes (S. Thyphimurium and S. Enteritidis) in chicken meat and giblets and also determination of antimicrobial resistance of the isolates.

Materials and Methods: In this study, livers (n=40), gizzards (n=40), hearts (n=30), skins (n=30), drumsticks (n=10) and wings (n=20) were collected from supermarkets and butcher shops in Konya, Turkey. The samples were analyzed by Classical Cultural Technique. Molecular confirmation of the suspicious colonies was carried out using Inv-A gene- based PCR. Flic-C and IE-1 primers were used by duplex PCR for S. Thyphimurium and S. Enteritidis respectively. Antibiotic resistance of the isolates was determined by the disk diffusion method.

Results: Forty-three (25.29 %) of 170 samples were positive for Salmonella spp. According to the d-PCR assay, neither S. Thyphimurium nor S. Enteritidis was not detected. The resistance to clindamycin, oxacillin, teicoplanin were evident 100 % and resistance to vancomycin (79.1 %), erythromycin (79.1 %), nalidixic acid (65.1 %), penicillin G (60.5 %) cephalothin (48.8 %), sulfamethoxazole-trimethoprim (37.2 %), tetracycline (37.2 %), ampicillin (23.3 %), kanamycin (18.6 %), chloramphenicol (11.6 %) amikacin, cephazoline, ciprofloxacin, gentamycin (4.7 %) was also detected. All isolates were susceptible to amoxicillin/ clavulanic acid and cefixime.

Conclusion: The results indicated that S. Enteritidis and S. Typhimurium were not identified and it was considered satisfactory in terms of public health. It should be still important to note the studies to identify species with lower pathogenic incidences for legal legislation. Furthermore, even the most common pathogenic species cannot be detected, the results of antibiotic resistance in isolates were noteworthy for antibiotic surveillance database.