pISSN:1309 - 6958       eISSN:2146 - 1953
2014, Cilt 30, Sayı 2, Sayfa(lar) 048-052
The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates
Şükrü Dursun1, Mehmet Köse2, Mesut Kırbaş1, Bülent Bülbül1, Seyit Ümütlü1
1Bahri Dağdaş Uluslararası Tarımsal Araştırma Enstitüsü, Konya, Türkiye
2Dicle Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Diyarbakır, Türkiye
Keywords: Thawing-transfer interval, embryo, transfer, cows, pregnancy rate
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Aim: In this study, it was aim to determine the rates of the effect of the lasting time from thawing-transfer until to transfer during transferring of cattle frozen embryos with ethylene glycol by direct transfer method on pregnancy rates.

Materials and Methods: Recipients (n=56) were selected from the cows whose estrus were synchronized with two times injections of 0.150 mg d-cloprostenol 14 days intervals. Embryos were transferred into recipients 7 days after the detection of recipients estrus (estrus= day 0). After embryos were threated 25 seconds within 25oC water bath to thaved then they were transferred to the recipients in minutes as follows; 0-5 (Group 0-5, n=27), 6-10 (Group 6-10, n=16) or 11-25 (Group 11-25, n=13) which all embryos were transferred into the uterine horn ipsilateral to the corpus luteum. Pregnancy diagnosis were done on day 28 used by ultrasound.

Results: Pregnancy rates were determined in 59.3%, 50% and 23.1% within G 0-5, G 6-10 and G 11-25 groups, respectively. Therefore, pregnancy rates were not statistically significant (P>0.05) between G 0-5 & G 6-10 with G 6-10 & G 11-25 while it was lower than the group of G 11-25 compared to the group of G 0-5 (P<0.05).

Conclusions: According to this study, frozen embryos by ethylene glycol should be transferred into recipients in within 10 minutes after thawing is concluded.