pISSN:1309 - 6958       eISSN:2146 - 1953
2008, Cilt 24, Sayı 1, Sayfa(lar) 087-091
Paternity Testing in Horses by Using Microsatellite DNA Markers: A Case Report
Yusuf Özşensoy1, Ercan Kurar1, Zafer Bulut2, Mehmet Nizamlıoğlu2
1Selçuk Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, KONYA
2Selçuk Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, KONYA
Keywords: Horse, DNA, Potermity testing, Microsatelit markar
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Parentage testing is critically important in horse breeding for actual evaluation of an animal, stud registrations, forensic studies and scientific researches. For this reason, DNA technology is widely used. The objective of this study is to determine genetic relationship between a mare and a foal by using blood and hair samples that were sent by the Attorney Generalship of Kars Province. DNA isolations were performed by using blood and hair samples. DNA samples were amplified at 10 horse microsatellite loci which were selected from a list suggested by International Society of Animal Genetics (ISAG). The resulting polymerase chain reaction (PCR) products were separated by capillary electrophoresis using a Beckman Coulter CEQ- 8000 Genetic Analysis System. Allele scoring was accomplished based on their base-pair size by fragment analysis and genotypes were determined each loci. Genotype data were evaluated based on common alleles. PCR products and so allele peaks were not determined for hair sample-2. When this sample was examined in detail, no hair roots were observed, which suggested that sampling was performed by a scissor. The same genotypes were observed between hair sample-1 and blood sample-2, therefore they belonged to the same animal. At least one common allele was observed at each AHT04, HMS02, HMS03, HTG06 and VLH20 loci for blood samples 1 and 2. On the other hand, common alleles were not determined for AHT05, HMS06, HTG07 and HTG10 microsatellite loci. These results suggested that no paternity is possible between the mare and the foal.