Aim: The aim of this research was to determine the effect of Corynebacterium cutis lysate on cytokine levels including hemogram and routine biochemical parameters in sheep.

Materials and Methods: In the research, 10 male yearling Merino sheep was used. Recommended dose (8 mg, 0.4 mL) of Corynebacterium cutis lysate (Ultra-corn® Inj.) was administered subcutaneously at a single dose to each animal. Before (0 hour, control) and after the treatments, blood samples were obtained from v. jugularis at 2, 4, 8, 12, 24, 48, 72 and 96 hours. Concentrations of serum Tumor necrosis factor alpha (TNFα), Interleukin (IL)-1β, IL-6 and IL-10 were determined with ELISA reader. Hemogram values (White blood cell, red blood cell, platelet, hematocrit, hemoglobin) were determined with hemocell counter, whereas biochemical values (Creatine kinase-MB, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, total protein, albumin, blood urea nitrogen, creatinine) were analyzed with autoanalyzer.

Results: Corynebacterium cutis lysate caused fluctuations on the cytokine levels (P>0.05). Hemogram parameters did not change statistically significant, while fluctuations were determined (P<0.05) in some biochemical values (Alkaline phosphatase, total protein, albumin, creatinine).

Conclusion: It may be stated that treatment of Corynebacterium cutis lysate has no impressive effect on the cytokine levels, and detailed researches are need to determine the effect of Corynebacterium cutis lysate on the passive immunity.