The aim of this study was carried out to determine the presence of Bovine Herpes Virus-1 (BoHV-1) in aborted small ruminant foetus' tissues by using Polymerase Chain Reaction (PCR) technique. For this purpose, aborted tissue samples (liver, lung, and spleen, totally n=198) were collected from small ruminants (43 sheep and 23 goats), each from different flocks and lived together with BoHV-1 seropositive cattle, in Central Anatolia region (Konya, Aksaray, and Niğde provinces) of Turkey. Glycoprotein D (gD) gene based PCR was used for the detection of BoHV-1. All tissue samples were detected as negative for BoHV-1 DNA. According to results, it can be expressed that BoHV-1 is not play a role in abortion cases in small ruminants in Central Anatolia Region.