pISSN 1309-6958
eISSN 2146-1953
| 2016, Cilt 32, Sayı 3, Sayfa(lar) 193-199 |
| [ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ] |
| Basal medium eagle solution may improve the post-thaw parameters of Kangal dog semen |
| Sukru Gungor1, Mustafa Numan Bucak2 |
| 1Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey 2Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey |
| Keywords: BME, ethylene glycol, glycerol, Kangal dog semen |
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Aim: The aim of this study was to investigate the effects of
BME on Kangal dog sperm parameters following the freezethawing
process.
Materials and Methods: Ejaculates were collected by digital manipulation twice a week. Ejaculates divided into six equal aliquots, and diluted with Tris-based extender (T) containing 5% glycerol (G) + BME 2.5% (TGB2.5), TG + BME 10% (TGB10), 5% ethylene glycol (E) + BME 2.5% (TEB2.5), TE + BME 10% (TEB10), TG and TE. Subsequently, the ejaculates were cooled to 4°C at 1.5 hours, and stored in liquid nitrogen (~-196°C). Results: The extender supplemented with 10% BME + 5% G resulted in higher progressive motility (23.19%), in comparison to the extenders supplemented with 5% EG (14.08%). Better sperm acrosome integrity was achieved with the use of the extender containing 2.5% BME + 5% G (44.99%), when compared to the use of the extender supplemented with 5% G (33.63%) (P<0.05). Conclusion: BME added to extender, may improve the postthawed sperm parameters on freezing of Kangal dog semen. |
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