The present report describes a clinical case of emphysematous abomasitis in lambs caused by Sarcina spp. The lambs were aged from the 24th hour after birth to the 35th day additionally supplemented with milk replacer due to the reduced amount of milk in the mothers, and from the second week they began to accept "ad libitum" starter granulated feed and ground corn. After which they began to show the following signs: severely swollen abdomen and difficult breathing, cachexia, lying down, lethargy, pale conjunctivae, feces were pale yellow mixed with mucus, internal body temperature was normal. In a period of 10 days, 50 lambs became ill and 10 died. At the performed autopsy, the abomasum was filled with gases, its contents had a whitish color and mucous consistency. The mucosa and submucosa were diffusely hyperemic, and had an edematous and emphysematous appearance. On the surface of the mucosa, desquamated epithelial cells mixed with exudate containing fibrin threads, numerous degenerated neutrophils, eosinophils, macrophages and grouped bacteria of the genus Sarcina spp. were observed. The combined use of pathoanatomical and histopathological examination are effective and reliable methods for diagnosis and differential diagnosis of emphysematous abomasitis in lactating lambs caused by bacteria of the genus Sarcina. Characteristic morphologies of bacteria allows us to distinguish them histologically from clostridia, as well as from other etiological agents