This study was carried out to reveal the origin, course and drainage area of the lingual vein and its branches in water buffaloes. For this purpose, a total of eight buffalo heads of both sexes, obtained from slaughterhouse, were used. Lingual vein started into a common trunk which was formed by four branches of sublingual vein to the deep lingual vein. The lingual vein that coursed caudoventrally after its origin passed lateral to the hypoglossal muscle and received the hyoidal arch at the middle of this muscle. The lingual vein inclined a caudodorsal direction and joined the facial vein at the level of the vascular notch. Except for sublingual vein, the vessels forming the deep lingual vein drained the lingual muscles (mm. linguae) apart from the hyoglossal muscle and the lingual root portion of the genioglossal muscle. The latters and the caudal part of the ge-niohyoideal muscle were drained by the hyoidal arch and some branches participating directly the lingual vein. The sublingual vein drained the mucous membrane of the floor of the oral cavity, the inciciv part of the mandibula, the lingual gland and also the cranial part of the geniohyoideal muscle.