The aim ol this study was to determine effectiveness of inactivated Salmonella Typhimurium vaccines ad-luvantod wilh mineral oil (MO), mineral 08 +■ ginseng (MO+G). aluminium hydroxide (AH) and aluminium hydroxide + ginseng (AH+G) in sheep and mice. S. Typhimurium vaccines were administered in sheep twice at 3 weeks intervals, subcutaneously or intramascularly Blood samples were collected at weeks 3, 5 and 9 after vaccination. The titers ol antibodies against S. Typhimurium in serum samples were detected by using self-made Enzyme Linked Immunosorbent Assay (ELI S A). The inactivated S, Typhimurium vaccines were administered to mice twice with two weeks intervals by IM or SC routes. The measurement of the antibody in mice was done by micro serum agglutination test (mSAT), Levels of antibodies in sera of vaccinated animals were significantly higher than the controls. No mortality and morbidity were observed m mice vaccinated and challanged. In addition, interactions between classical adjuvants and ginseng wore also evaluated. Consequently, effectiveness of S, Typhimurium vaccines prepared from local strains was found to bo useful to protect sheep from the infection ol S. Typhimurium.