The purpose of this study was to assess the carriage of Clostridium difficile in household pets to determine their potantial as a reservoir of infection. Feacal samples from dogs and cats were cultured in Cooked-Meat Broth. The cultures grown were inactivated in terms of vegetative bacteria in water bath for 20 min. at 80"C. Then, the cultures were subcultured on selective Colombia Blood agar plates containing gentamisin (5 mg/L). Agar plates were incubated aerobically and anaerobically for 48 h. at 37°C. Identification was based on colony morphology, flouresence under an ultraviolet lamb, Gram staining, subterminal spor formation, and some biochemical properties. Clostridium difficile strains were isolated from faecal samples of adults dogs and puppies as household pets: 17 positive (% 25.95) of 62 samples and 9 positive (% 28.1) of 32 samples, at ratio respectively. In uncontrolled dogs (both adults or puppies), 7 positive (% 14.28) of 49 samples and 6 positive (% 19.2) of 37 samples were determined, In cats, 4 positive (% 25) of 16 samples were detected. The strains of C. difficile were found to be susceptible to antimicrobial agents, as follows; Enrofloxacin (100 % ), Oxytetracyclin (100 % ), Danofioxacin (97.6 %), Erithromycin (90.6 % ), Penicilin (85.5 % ), Nitrofurazolidone (83.7 % ), Amoxycillin (69.7 % ) and Nalidixic Acid (62.7 % ).