Aim: The aim of the study was to detect the gross morphology and measurement of the length of chordae tendineae of bovine atrioventricular heart valves for future bioprosthesis.

Materials and Methods: Forty atrioventricular heart valves were obtained from cattle. The tricuspid and bicuspid valves were harvested by opening the four chambers of the collected heart specimen by placing finger tightly into the cusps of the valves and then the ventricular myocardium muscle trimmed out by scissor slowly. Harvested valves were gently packed with cotton wool to maintain the normal architecture of the leaflets and then preserved in 10% formalin solution.

Results: A strongly significant (p<0.01) difference was found amongst the age group of anterior, intermediate and posterior cusps of the tricuspid valves. It's also same for the posterior cusp of the bicuspid valve (p<0.01), although a statistically significant (p<0.05) difference was found for anterior cusps of the bicuspid valves. The mean length of the chordae tendineae of anterior cusp of 2.5 years age group was higher than posterior cusp.

Conclusion: The arrangement of the valvular apparatus, the close relation tricuspid and bicuspid valves and adjacent structures like papillary muscles, chordae tendineae and atrioventricular conduction bundle are all important in the clinical arena for future bioprosthesis and other vital purposes.