pISSN:1309 - 6958       eISSN:2146 - 1953
2010, Cilt 26, Sayı 1, Sayfa(lar) 015-019
Effects of drugs on kinetic values of cytokines, adenosine deaminase and 13,14-dihydro-15-keto-prostaglandin F in endotoxemia: A different approach
Feray Altan1, Muammer Elmas2, Ayse Er2, Kamil Uney2, Gul Cetin3, Bunyamin Tras2, Enver Yazar2
1Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Dicle, Campus, Diyarbakir, Turkey
2Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Campus, Konya, Turkey
3Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, Turkey
Keywords: Enrofloxacin, flunixin, dexamethasone, kinetic, endotoxemia markers
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Aim: The objective of this study was to determine the effects of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone (DEX) on kinetic values of cytokines, adenosine deaminase (ADA) and 13,14-dihydro-15-keto-prostaglandin F2a (PGM) in lipopolysaccharide-induced endotoxemia. Materials and Methods: Rats were divided into seven groups. To induce endotoxemia, lipopolysaccharide (LPS) was injected into all groups, including the positive control. The six other groups received the following drugs: ENR, FM, low-dose DEX, high-dose DEX, ENR + FM + low-dose DEX and ENR + FM + high-dose DEX. After the treatments, blood samples were collected at 0, 1, 2, 4, 6, 8, 12, 24, and 48 hours. Serum tumor necrosis factor alpha (TNFα), interleukin- 6 (IL-6), interleukin-10 (IL-10), ADA and plasma PGM levels were determined by ELISA. Area under the concentration time curve (AUC0-48), maximal concentration in plasma or serum (Cmax) and time to reach (tmax) values were determined by pharmacokinetic computer program. Results: ENR increased (p<0.05) AUC0-48 of PGM and decreased (p<0.05) AUC0-48 of IL-6, IL-10 and ADA, while FM decreased (p<0.05) AUC0-48 of IL-6 and ADA compared to LPS group. DEX alone and combined administrations caused the lower AUC0-48 of all values (p<0.05). Conclusion: Kinetic values, especially AUC, may be used for total evaluation of endotoxemia markers determined at different sampling times in same groups as a different and logical approach.