Aim: This study was aimed to identify of coagulase negative staphylococci (CNS) isolated from bovine milk samples with subclinical mastitis and to determine susceptibility of microorganisms to antibiotics.

Materials and Methods: Totally, 286 CNS isolates from milk samples were identified according to colony morphology, haemolysis and biochemical properties.

Results: Out of isolates, 40 (14.38%) were identified as S. simulans, 31 (11.15%) S. epidermidis, 54 (19.42%) S. chromogenes, 31 (11.15%) S. xylosus, 22 (7.91%) S. caprae, 19 (6.83%) S. warneri, 18 (6.47%) S. haemolyticus, 13 (4.67%) S. cheiferi subsp. coagulans, 10 (3.59%) S. cohneii subsp. urealyticus, 10 (3.59%) S. saprophyticus, 6 (2.15%) S. gallinarum, 7 (2.51%) S. hominis, 4 (1.43%) S. hyicus, 2 (0.71%) S. cheiferi subsp. cheiferi, 9 (3.23%) S. lentus and 2 (0.71%) S. sciuri. The susceptibilities of CNS were determined to be variable to different antimicrobial agents.

Conclusions: This study was shown that identification of CNS isolated from dairy cows with subclinical mastitis should be strictly performed.