The aim of this research was to investigate Enterobius vermicularis contamination in playgrounds and public transportation vehicles in Afyonkarahisar province. The research was conducted in randomly selected 38 public transportation vehicles providing service in the city centre and in randomly selected 9 playgrounds. E. vermicularis eggs were not detected in any of the 126 samples taken from the playgrounds using the cellophane tape method. On the other hand, the examination of the 150 samples taken from public transportation vehicles by the same method demonstrated the presence of E. vermicularis eggs in 2 (1.33%) of the samples. It was concluded that public transportation vehicles may play a role in the transmission of E. vermicularis. Therefore, hygiene conditions should be improved in public areas.