An 8 years old male Anatolian shepherd dog (ASH) was referred to the Small Animal Hospital of the Faculty of Veterinary Medicine of Selçuk University with a history of cardiac murmur. The diagnosis was asymptomatic degenerative mitral valve disease. The severity of regurgitation was quantified by the maximal ratio of the regurgitant jet area signal by color Doppler to Left atriun area (ARJ/LAA), the proximal isovelocity surface area (PISA) and the subtraction methods by Doppler examinations. The each quantification methods was valuable to assess the severity of disease in ASH with MR.