Aim: In recent years, corn syrup and trans fats have an important place in food production and consumption. Since trans fats (TF) and high fructose corn syrup (HFCS) can cause similar negative effects on health, the aim of this study was to determine the changes in some biochemical parameters of an organism following the intake of HFCS and TF in the diet.

Materials and Methods: Wistar Albino male rats (n = 24) were divided 4 equal groups. Control group (C) was fed with a standard rat feed, 2nd group with cotton fat, 3rd group with trans fat, and 4th group with trans fat and corn syrup for 8 weeks. Blood samples were collected on day 0 by the supraorbital method and on day 60 by the intracardiac method under anesthesia. Glucose, HbA1c, triglyceride, cholesterol, HDL, LDL, AST, ALT, ALP, SOD, and GGT analyzes were performed.

Results: In the present study, trans fats increased triglyceride concentration, decreased HDL concentration and caused changes in some liver enzyme values. Increase in glucose and triglyceride concentration and decrease in HDL and GGT analyzes were statistically significant (p < 0.05). The increase and decrease in LDL, AST and HbA1c analyzes were not statistically significant (p > 0.05).

Conclusion: High fructose corn syrup and trans fatty acid found in foods ıt was concluded that the amount should be limited as much as possible, and these limitations will positively affect human health.