pISSN:1309 - 6958       eISSN:2146 - 1953
2022, Cilt 38, Sayı 4, Sayfa(lar) 225-231
Ellagic acid could prevent experimentally induced oxidative stress in rats
Mehmet Ali Kisacam1, Gonca Ozan Kocamuftuoglu2, Enver Ozan3, Sema Temizer Ozan4
1Mustafa Kemal University, Veterinary Faculty, Department of Biochemistry, Hatay, Turkey
Mehmet Akif Ersoy University, Veterinary Faculty, Department of Biochemistry, Burdur, Turkey
3Firat University, Medicine Faculty, Department of Histology and Embryology, Konya, Turkey
4Firat University, Veterinary Faculty, Department of Biochemistry, Konya, Turkey
Keywords: Diethylnitrosamine, ellagic acid, oxidative stress, phenobarbital
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Aim: Reactive oxygen species (ROS) are produced during the metabolism of diethylnitrosamine (DEN) and phenobarbital (PB). Ellagic acid (EA) is a natural biologically active polyphenol with antioxidant activity. The present study was conducted to assess the preventive and protective capability of EA on DEN-PB-induced oxidative damage in rats.

Materials and Methods: 60 Sprague-Dawley rats were assigned into 6 groups: Negative control, DEN, EA, DEN-PB (positive control), DEN-PB-EA (treatment), and EA-DEN-PB (pretreatment). 150 mg/kg DEN were administered to DEN, positive control, treatment and pretreatment groups. After two weeks, 500 ppm PB applied to positive control and treatment groups. 50 mg/kg/day EA were orally given to EA, and pretreatment groups for 8 weeks and treatment group was given for four weeks. After 8 weeks rats were sacrificed, liver and blood were subjected to biochemical evaluation.

Results: DEN alone or with PB increased liver MDA levels significantly; however plasma MDA levels didn?t change. EA pretreatment decreased liver MDA levels (p<0.001). CAT activity did not change in the liver and erythrocytes of the positive control group. Liver CAT activity increased insignificantly in pretreatment and significantly in treatment group (p<0.05). SOD activity did not change in liver and erythrocytes of DEN and positive control groups. EA increased liver SOD activity significantly (p<0.001). DEN application alone or with PB decreased liver and blood GSH levels significantly (p<0.05), while EA application increased liver GSH levels in treatment (p<0.05). Blood GSH levels decreased significantly in treatment (p<0.001).

Conclusion: It is considered that EA pretreatment could be more effective than EA treatment in DEN, PB induced oxidative stress in rats.