Aim: This study was conducted to investigate the effect of diallyl disulfide, one of the bioactive compounds of garlic, on the malondialdehyde level of colon tissue in rats with azoxymethane-induced colon cancer.

Materials and Methods: A total of 58 male Wistar Albino rats were used in the study. The animals were divided into 5 groups. The first group was the control group (n=12), the second group was the cancer group (n=12) treated with azoxymethane (15 mg/kg body weight), the third group was the group to whom only diallyl disulfide (DADS, 50 mg/kg body weight) was administered (n=11), the fourth group was the group to whom azoxymethane and diallyl disulfide were administered (n=11) and the fifth group was the group to whom corn oil (1 ml/kg body weight) was administered as carrier substance (n=12). The experimental the one which period at the end of 18 weeks, the animals were sacrificed by cervical dislocation and their colon tissues were excised. The tissues were homogenized and MDA levels were measured with the ELISA device using the procedure, suitable for the kit.

Results: Tumor formation in the tissues was macroscopically identified. The analysis results indicated no difference between the groups with respect to their MDA levels (P>0.05).

Conclusion: In conclusion, it can be asserted that the administration of DADS or azoxymethane did not cause lipid peroxidation in the rat colon tissue under the experimental conditions. There is a need for further studies investigating the effectiveness of DADS or AOM in colon tissue by taking the method and the dose of treatment to the animals into consideration.