Four hundred sixty nine milk and serum samples from healthy Holstein dairy catties at the age over three years old and were in the late pregnancy period, were tested for Bovine Leukaemia Virus (BLV) antibody by ELISA-milk and AG ID-blood in Burdur province. Of 469 serum - milk samples, 23 (4.9 %) and 90 (19.2 %) were found to be positive by AGID test and ELISA, respectively. The lowest number (1) of seropositive animals was detected in Burdur-Merkez M3. Burdur-Yeşilova Y1, Y2, Burdur-Tefenni T2, T3 and the highest number (11) of seropositive animals was determined in Bur-dur-Merkez M2 by AGID. No seropositive animals were found in Burdur-Merkez M1 and Burdur-Ağlasun A1, The lowest number (3) of seropositive animals was detected in Burdur-Yeşilova Y1 and the highest number (17) of seropositive animals was detected in Burdur-Tefenni T1 by ELISA. By both AGID and ELfSA. the most BLV infected animals were found in Burdur-Merkez M2, Burdur-Telenni T1 and Burdur-Ağlasun A2. It was revealed that mix or common suckling of catves, extensive treatment efforts before artificial insemination and frequent rectal palpation were common procedures in the farms which had the highest prevalence scores for BLV.