The aim of this study was to investigate the clinicopathologic and pathologic features of experimentally induced acute pancreatitis in dogs. Total 13 healthy dogs were used and grouped as experimental group (7 dogs) and control group (6 dogs). After laparatomie, oleic acid (0.5 ml/kg body weight) was injected into pancreas of experimental group dogs. Clinically, the experimental group dogs were showed fever, anorexia, depression, vomiting and steatorrhea. The median serum cholesterole and trigliseride concentrations, median serum amylase and lipase activities, and urine amylase/creatinine ratio were increased in the experimental group when compared with the values of control group dogs. The median serum ALP activities were increased, whereas the median serum ALT activities did not show any significant alteration. Pathologically, haemorrhagic-necrotic pancreatitis, thrombosis of blood vessels, fat necrosis and in some cases abscessation were detected in the pancreas of experimental group dogs. In conclusion, the combination of serum lipase enzyme activity with urine amylase/creatinine ratio, might be useful in the diagnosis of acute pancreatitis.