Aim: This trial was carried out to investigate the effects of glycerol which was given to dairy cows in the transition period milk yield and composition, live weight, and body condition scores with changes in blood plasma glucose, nonesterified fatty acid (NEFA) and ß hydroxybutyric acid (BHBA) concentrations.

Materials and Methods: Twenty of second calf pregnant 3 years old Holstein cows were used. Cows were divided into 2 groups, one kept as a control, and second group was drenched an extra 750 g glycerol daily. The experiment has been continued total of 65 days with prenatal 20 days and postnatal 45 days.

Results: There was no significant difference in terms of milk yield, milk composition, live weight, and plasma glucose and BHBA concentrations between the groups (p>0.05). 45^th day plasma NEFA concentrations were found lower in the glycerol group than the control group (p<0.05).

Conclusion: The results indicate that glycerol has a beneficial effect on plasma and live weight changes of cows in transition period.