The aim of the study was to determine histological structure of both nictitating membrane, itself and, its glands, to evaluate possible contribution to the local immunity of the eye itself and the basic histological knowledge to be used as reference out on local interventions. The periodic acid-Schiff (PAS), alcian blue pH=2.5 (AB) and PAS/AB at pH=2.5 combined staining was used to histochemical properties in the glands of the palpebra tertia (PT). It was observed that the PT of Akkaraman sheep and ram investigated had an T" shaped hyaline cartilage, so-called cartilago palpebra tertia (KPT) which was covered with the continuation of conjunctival epithelial tissue in the medial canthus in Akkaraman sheep and ram. The epithelia of the both palpebral and bulbar faces, and free marginal aspect of the PT were stratified squamous epithelium as in conjunctiva. Gil. palpebra tertia superficialis (GPTS) was observed as lobes surrounded by connective tissue capsule located around the KPT. Varying degrees of pigmentation was observed over the free margin, palpebral and bulbar aspects of the PT in Akkaraman sheep and ram.