In this study the effects of vitamin A, E and C supplemented diet of laying hens on serum total protein, albumin, glucose, Ca and P values were investigated. As material a total of 2160 that are 1728 Hisex Brown, 216 Hyline-W77, 216 Babcock-B300 egg type chickens in 26 weeks of age were used and these animals divided in ten groups each has 216 chicken. Blood samples were taken from these animals once a moth in which 1,2, 4 and 7. month. Total protein, albumin, glucose, Ca and P values of sera obtained from each group were analyzed by using spectrophotometer. As a result, because of differentiation is not specific statistically between vitamin negative and positive groups, vitamin levels in the diet based corn and soybean meals were thought to be enough for animals without factories that effects vitamin necessity of the animals such as diseases and heat stress. At these situations, it may said that the vitamin supplementation in high ratio to the diet is not necessary according to analyzed parameters.