Materials and Methods: The study was conducted on 8 bitches with TVT. . The autogenous vaccine applied on the dogs was maddened the based on the bovine papillomatosis vaccine. Approximately 24 g of tumor tissue and 48 mL Tris buffer (pH 7.5) solution were crushed in cold conditions (+4 0C) during 15-20 minutes in mortar disintegrated with autoclaved sterile sand. Half of the mixture (36 mL) was completed with tris buffer to 60-65 mL. 0.5 percent formalin was added this mixture and mixture was portioned to 6 each has 10 mL. The prepared vaccine was administered 4 times (SC) with an interval of 1 week. Dogs were followed up 3 months after 4th application and evaluations were performed for tumor regression.

Results: Serosanguineous or hemorrhagic genital discharge was determined in dogs prior to application of autogenous vaccine. The lesions in external genital organs are like cauliflower, rubescent, fresh-looking and easily bleed on touched. After administration of the autogenous vaccine, serosanguineous or hemorrhagic genital discharge from the masses on the external genital organs of dogs continued, and there were no changes in the mass fragility. After the application of autogenous vaccines at four times, there was no any decline and improvement in the masses on the vagina and vulva.

Conclusions: It is believed that a viral agent may contribute to the etiology of TVT. Prepared autogenous vaccine technique used in the treatment of bovine papillomatosis derived from bovine papilloma virus may not be suitable in the treatment of bitches with TVT.