pISSN:1309 - 6958       eISSN:2146 - 1953
2013, Cilt 29, Sayı 2, Sayfa(lar) 092-096
Comparison of various techniques used for diagnosis of rabies in cats
Mugale Madhav Nilakanth1, Bhupinder Singh Sandhu2, Beigh Akeel2, Gupta Kuldeep3, Sood Naresh Kumar2, Charan Kamal Singh2
1Department of Veterinary Pathology, Madras Veterinary College, Vepary, Chennai, Tamilnadu
2Department of Veterinary Pathology, GADVASU, (PAU campus) Ludhiana, Punjab, India
Keywords: Brain, direct fluorescent antibody test, histopathology, immunohistochemistry, rabies
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Aim: Objectives of this study was to compare and evaluate the best method for diagnosis of rabies in cats.

Materials and Methods: Antemortem examination of 5 suspected cats were evaluated. Brains were collected from the suspected cats. Direct Fluorescent Antibody Test (dFAT), Hematoxylin and Eosin (H & E) and Immunohistochemistry (IHC) histopathological diagnostic tests were applied.

Results: The dFAT test was conducted on the saliva and fresh brain impression smear of all cats, among that 4 cats showed positive for rabies virus. After death of cats, Hematoxylin & Eosin (H&E) and Immunohistochemistry (IHC) techniques were carried out. One case showed meningitis and remaining showed Negri bodies. Viral antigen depositions were observed by counting 100 cells each region of hippocampus and cerebellum by H&E and IHC. Hippocampus pyramidal cell showed 87% and cerebellum Purkinje cells showed 69% of rabies viral antigen deposition.

Conclusion: The IHC can be used as reliable diagnostic technique in addition to dFAT. IHC shows positivity in mildly infected cases and having immense value for retrospective studies. It also minimizes the risk of public health hazard during shipping of rabid positive brain samples.